Download Advances in Macromolecular Carbohydrate Research, Volume 1 by R.J. Sturgeon PDF

By R.J. Sturgeon

In recent times the main major advances in carbohydrate learn were made within the wisdom of the constitution and serve as of carbohydrates within the macromolecular country. This name addresses these parts of the topic within which the authors think an important paintings is being conducted.

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Extra resources for Advances in Macromolecular Carbohydrate Research, Volume 1

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128] used affinity chromatography on divinylsulphonesubstituted Sepharose and chelating Sepharose (in the Cu^^form) to separate arabinanases and galactanases from A. niger (var. aculeatus). Chromatofocusing on Mono-P appears to be an efficient method to remove a persistent contamination of arabinofuranosidases from an e«^o-arabinanase from A. aculeatus (unpublished results). During fractionation of arabinanases a reliable, simple, and quick assay is needed to identify them. -nitrophenyl-a-L-arabinoside.

Niger strains [138,171,172]. The physicochemical properties of these enzymes, summarized in Table 17, are comparable to those of the previously examined ewtio-arabinanases (see Table 10). The action patterns of all recently purified Aspergillus endoarabinanases examined appear similar to that of the A. niger enzyme previously discussed [19,89] since arabinobiose and arabinotriose are the major final hydrolysis products released from linear arabinan [137,151,171,173]. However, comparison of the initial (transient) hydrolysis products released from this substrate by the A.

During studies of the enzymic degradation of alkali-extractable wheat-flour arabinoxylan, Kormelink et al. [146] examined the action of the A. niger arafur A on isolated arabinoxylan-derived oligosaccharides. While this enzyme was not active against polymeric arabinoxylan, it readily cleaved all (l-^3)-a-linked arabinofuranosyl groups from singly substituted xylopyranosyl residues in arabinoxylan oligosaccharides, irrespective of whether the substituted xylopyranosyl residue was in a terminal or nonterminal position.

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